Lentinus edodes the 2 nd 5 ' - Nucleotide - forming Nuclease from

28, 36, and 41 kDa, On the other hand, Kurosawa et al.4) found an alkaline RNase in addition to three acid nucleases in frcsh fruit bodies of L, edodes confirming the results of Endo et aLi) To establish more precise information on these enzymes, we tried to isolate and characterize each nuclease or RNase in L. edodes. First, we isolated an acid RNase, RNase Le2, and established the prirnary structure of this enzyme.6'7) It was a base non-specific RNase belonging to the RNase T, family. As fbr thc nuclease, in our previous paper we iselated an endonuclcases which is activatcd by Zn2'.6) The molecultir mass of this enzyrne was about 27 kDa so larjudged by SDS-PAGE. The N-terrninal amino acid sequence of nuclease Lei was very similar to those of Pl nuclease from Penicitlium citrinumS'9) and Sl nuclease from AspergiUus oryzae.iO) In this paper, to discover any further necleases responsible fbr the production of 5'-nucleotides and